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Goat Anti-SLC4A11 / BTR1 Antibody (EB09681)

Related Products: EBP09681 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09681 Goat Anti-SLC4A11 / BTR1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09681 1
EB09681 (0.3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09681 2
EB09681 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SLC4A11, solute carrier family 4, sodium borate transporter, member 11, BTR1, CDPD, CDPD1, CHED2, MGC126418, MGC126419, NABC1, dJ794I6.2, OTTHUMP00000030097, bicarbonate transporter related protein 1, sodium-coupled borate cotransporter 1, solute carrier family 4 member 11, solute carrier family 4, sodium bicarbonate transporter-like, member 11
Official Symbol: SLC4A11
Accession Number(s): NP_114423.1
Human GeneID(s): 83959
Non-Human GeneID(s): 269356 (mouse), 311423 (rat)

Immunogen

Peptide with sequence C-HTSRKYLKLKNFKEE, from the internal region of the protein sequence according to NP_114423.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90kDa band observed in Human Kidney lysates (calculated MW of 99.6kDa according to NP_114423.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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