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Goat Anti-SMAD4 / MADH4 Antibody - Trial (EB05149-T)

Related Products: EBP05149 - Immunizing Peptide, Goat Anti-SMAD4 / MADH4 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05149-T Goat Anti-SMAD4 / MADH4 Antibody - Trial Pep-ELISA, IP Yes 20µg specific antibody in 40µl $105.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
Click here to download PDF datasheet.

Target Protein

Principal Names: MADH4, SMAD4, JIP, DPC4, MAD, mothers against decapentaplegic homolog 4 (Drosophila), Mothers against decapentaplegic, Drosophila, homolog of, 4, MAD (mothers against decapentaplegic, Drosophila) homolog 4, SMAD family member 4, OTTHUMP00000163548, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4, mothers against decapentaplegic homolog 4
Official Symbol: SMAD4
Accession Number(s): NP_005350.1
Human GeneID(s): 4089
Non-Human GeneID(s): 50554 (rat)

Immunogen

Peptide with sequence C-HTMPIADPQPLD, from the C Terminus of the protein sequence according to NP_005350.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave a band at approx 75kDa in lysates of cell line NIH3T3. Please note that currently we cannot find an explanation in the literature

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig Cow

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