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Goat Anti-Snail homolog 1 / SNAI1 Antibody (EB07405)

Related Products: EBP07405 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07405 Goat Anti-Snail homolog 1 / SNAI1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07405 1
EB07405 (0.1µg/ml) staining of Mouse (A) and Rat (B) Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SNAI1, snail homolog 1 (Drosophila), SLUGH2, SNA, SNAH, dJ710H13.1, snail 1 (drosophila homolog), zinc finger protein, snail 1 homolog, snail 1 zinc finger protein
Official Symbol: SNAI1
Accession Number(s): NP_005976.2
Non-Human GeneID(s): 20613 (mouse), 116490 (rat)

Immunogen

Peptide with sequence RKPSDPNRKPNY-C, from the N Terminus of the protein sequence according to NP_005976.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 28kDa band observed in Mouse and Rat Kidney lysates (calculated MW of 29.1kDa according to Mouse NP_035557.1 and Rat NP_446257.1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

Specific Reference

This antibody has been successfully used in IHC and Western blot on Rat:
Cui F, Zou D, Gao Y, Zhang N, Wang J, Xu L, Geng J, Li J, Zhou S, Wang X.
Effect of Tongxinluo on Nephrin Expression via Inhibition of Notch1/Snail Pathway in Diabetic Rats.
Evid Based Complement Alternat Med. 2015;2015:424193
PMID: 26417374

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