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Goat Anti-Snail homolog 1 / SNAI1, Biotinylated Antibody (EB07405-B)

Related Products: EBP07405-B - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07405-B Goat Anti-Snail homolog 1 / SNAI1, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07405-B 1
Biotinylated EB07405 (0.1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: SNAI1, snail family transcriptional repressor 1, SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1, protein sna, protein snail homolog 1, snail 1 homolog, snail 1 zinc finger protein, snail 1, zinc finger protein, snail family zinc finger 1, snail homolog 1
Official Symbol: SNAI1
Accession Number(s): NP_005976.2
Human GeneID(s): 6615
Non-Human GeneID(s): 20613 (mouse), 116490 (rat)

Immunogen

Peptide with sequence RKPSDPNRKPNYC., from the N Terminus of the protein sequence according to NP_005976.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 28kDa band observed in lysates of cell line A431 (calculated MW of 29.1kDa according to NP_005976.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.03-0.1µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

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