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Code Name Applications Availability Size Tested species Price Grade  
EB06217 Goat Anti-SNX5 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06217 1
EB06217 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB06217 2
EB06217 (4µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SNX5, FLJ10931, sorting nexin 5, RP11-504H3.2
Official Symbol: SNX5
Accession Number(s): NP_055241.1; NP_001269383.1
Human GeneID(s): 27131
Non-Human GeneID(s): 69178 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_055241.1; NP_001269383.1). Reported variants represent identical protein: NP_055241.1, NP_689413.1

Immunogen

Peptide with sequence SLLQSCIDLFKNN, from the C Terminus of the protein sequence according to NP_055241.1; NP_001269383.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 48kDa band observed in Jurkat and A549 lysates (calculated MW of 46.8kDa according to NP_055241.1). Recommended concentration: 1-3µg/ml. Primary incubation was one hour.
IHC: In paraffin embedded Human Breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of Human Kidney and in trophoblasts of Human Placenta . Recommended concentration: 3-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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