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Goat Anti-Sorbitol Dehydrogenase Antibody (EB06601)

Related Products: EBP06601 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06601 Goat Anti-Sorbitol Dehydrogenase Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB06601 1
EB06601 (0.1µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: sorbitol dehydrogenase, SORD, SORD1
Official Symbol: SORD
Accession Number(s): NP_003095.2
Human GeneID(s): 6652

Immunogen

Peptide with sequence KIMLKCDPSDQNP, from the C Terminus of the protein sequence according to NP_003095.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 38kDa band observed in Mouse Kidney lysates (calculated MW of 38.3kDa according to NP_003095.1). Recommended concentration: 0.1-0.3µg/ml. A minor band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Niimi N, Yako H, Takaku S, Kato H, Matsumoto T, Nishito Y, Watabe K, Ogasawara S, Mizukami H, Yagihashi S, Chung SK, Sango K.
A spontaneously immortalized Schwann cell line from aldose reductase-deficient mice as a useful tool for studying polyol pathway and aldehyde metabolism.
J Neurochem. 2018 Mar;144(6):710-722.
PMID: 29238976

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