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Code Name Applications Availability Size Tested species Price Grade  
EB05455 Goat Anti-SORBS1 / Ponsin (C Terminus) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB05455 1
EB05455 (2.5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SORBS1, PONSIN, sorbin and SH3 domain containing 1, CAP, SH3D5, SORB1, SH3P12, ponsin, sh3p12, FLJ12406, KIAA1296, DKFZP586P1422, DKFZp451C066, SH3-domain protein 5 (ponsin), FLAF2, FLJ12406, R85FL, Fas-ligand associated factor 2, c-Cbl associated protein, DKFZp586P1422, OTTHUMP00000020142, OTTHUMP00000020143, OTTHUMP00000020144, OTTHUMP00000020145, ponsin
Official Symbol: SORBS1
Accession Number(s): NP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1
Human GeneID(s): 10580
Non-Human GeneID(s): 20411 (mouse)
Important Comments: This antibody is expected to recognize all seven reported isoforms (NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1 and NP_001030129.1).

Immunogen

Peptide with sequence C-GTFPGNYVKPLYL, from the C Terminus of the protein sequence according to NP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: No signal obtained yet but low background observed in Human Heart and 293 lysates at up to 2µg/ml.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig

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