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Goat Anti-SP1 Antibody (EB06834)

Related Products: EBP06834 - Immunizing Peptide, Goat Anti-SP1 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB06834 Goat Anti-SP1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06834 1
EB06834 staining (0.3µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SP1, Sp1 transcription factor, HGNC:11205, specificity protein 1
Official Symbol: SP1
Accession Number(s): NP_612482.2; NP_003100.1
Human GeneID(s): 6667
Important Comments: This antibody is expected to recognise both reported isoforms (NP_612482.2; NP_003100.1).

Immunogen

Peptide with sequence CSRIESPNENSNNSQ, from the internal region of the protein sequence according to NP_612482.2; NP_003100.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 100kDa band observed in lysates of cell lines HeLa (calculated MW of 80.7kDa according to NP_612482.2). Recommended concentration: 0.3-1µg/ml. Primary incubated for 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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