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Goat Anti-SP140 (aa29-41) Antibody (EB12527)

Related Products: EBP12527 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12527 Goat Anti-SP140 (aa29-41) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12527 1
EB12527 (2µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SP140, SP140 nuclear body protein, LYSP100, LYSP100-A, LYSP100-B, lymphoid-restricted homolog of Sp100, lymphoid-specific SP100 homolog, nuclear autoantigen Sp-140, nuclear body protein SP140, speckled 140 kDa
Official Symbol: SP140
Accession Number(s): NP_009168.4; NP_001005176.1; NP_001265380.1; NP_001265381.1; NP_001265382.1
Human GeneID(s): 11262
Important Comments: This antibody is expected to recognize all reported isoforms (NP_009168.4; NP_001005176.1; NP_001265380.1; NP_001265381.1; NP_001265382.1).

Immunogen

Peptide with sequence EGQNLQEQVCPEP, from the N-Terminus of the protein sequence according to NP_009168.4; NP_001005176.1; NP_001265380.1; NP_001265381.1; NP_001265382.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 140kDa band observed in Human Spleen lysates (calculated MW of 98.2kDa according to NP_009168.4). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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