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Goat Anti-SPAG16 (aa139-152) Antibody (EB09826)

Related Products: EBP09825 - Immunizing Peptide, EBP09826 - Immunizing Peptide, Goat Anti-SPAG16 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09826 Goat Anti-SPAG16 (aa139-152) Antibody Pep-ELISA, FC, IF, IHC Yes 100µg specific antibody in 200µl Human, Mouse $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB09826 1
EB09826 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09826 2
EB09826 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: DKFZp666P1710, FLJ22724, FLJ37717, MGC87036, PF20, sperm associated antigen 16, sperm-associated WD repeat protein, WD repeat domain 29, WDR29, SPAG16
Official Symbol: SPAG16
Accession Number(s): NP_078808.3; NP_001020607.1
Human GeneID(s): 79582
Important Comments: This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).

Immunogen

Peptide with sequence C-KGVTELRTVGNVPD, from the internal region of the protein sequence according to NP_078808.3; NP_001020607.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: This antibody was successfully used in Human and Murine frozen Brain sections (PMID: 25086173).

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Dog, Cow

Specific Reference

de Bock L, Somers K, Fraussen J, Hendriks JJ, van Horssen J, Rouwette M, Hellings N, Villar LM, Alvarez-Cermeño JC, Espiño M, Hupperts R, Jongen P, Damoiseaux J, Verbeek MM, De Deyn PP, D'hooghe M, Van Wijmeersch B, Stinissen P, Somers V.
Sperm-associated antigen 16 is a novel target of the humoral autoimmune response in multiple sclerosis.
J Immunol. 2014 Sep 1;193(5):2147-56.
PMID: 25086173

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