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Goat Anti-SPEN / SHARP Antibody (EB07168)

Code Name Applications Availability Size Tested species Price Grade  
EB07168 Goat Anti-SPEN / SHARP Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

Target Protein

Principal Names: SPEN, spen homolog, transcriptional regulator (Drosophila), KIAA0929, MINT, RP1-134O19.1, SHARP, Msx2 interacting nuclear target (MINT) homolog, OTTHUMP00000044600, SMART/HDAC1 associated repressor protein, nuclear receptor transcription cofactor spen homolog, transcriptional regulator
Official Symbol: SPEN
Accession Number(s): NP_055816.2
Human GeneID(s): 23013

Immunogen

Peptide with sequence C-DSEGKMDDKKEDHKE, from the internal region of the protein sequence according to NP_055816.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of approx. 402kDa according to NP_055816.2. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human

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