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Goat Anti-SPHK1 (C-term.) Antibody - Trial (EB05266-T)

Related Products: EBP05266 - Immunizing Peptide, EBP08409 - Immunizing Peptide, Goat Anti-SPHK1 Antibody, Goat Anti-SPHK1 (C-term.) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05266-T Goat Anti-SPHK1 (C-term.) Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human $105.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05266-T 1
EB05266 (1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SPHK1, sphingosine kinase 1, SPHK
Official Symbol: SPHK1
Accession Number(s): NP_068807.2; NP_892010.2; NP_001136073.1
Human GeneID(s): 8877
Important Comments: This antibody is expected to recognize all reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). The variants NP_001136073.1 and NP_001136074.1 represent identical protein.

Immunogen

Peptide with sequence C-SWKPQQMPPPEEPL, from the C Terminus of the protein sequence according to NP_068807.2; NP_892010.2; NP_001136073.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50kDa band observed in Human Lung and Placenta lysates (calculated MW of 51.1kDa according to NP_892010.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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