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Goat Anti-SPINT2 Antibody (EB10237)

Related Products: EBP10237 - Immunizing Peptide, EBP10238 - Immunizing Peptide, Goat Anti-SPINT2 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10237 Goat Anti-SPINT2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10237 1
EB10237(0.3µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10237 2
EB10237 (3.75µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: DIAR3, FLJ45571, HAI2, HAI-2, hepatocyte growth factor activator inhibitor type 2, Kop, PB, placental bikunin, serine peptidase inhibitor, Kunitz type, 2, serine protease inhibitor, Kunitz type, 2, SPINT2
Official Symbol: SPINT2
Accession Number(s): NP_066925.1; NP_001159575.1
Human GeneID(s): 10653
Important Comments: This antibody is expected to recognize both reported isoforms (NP_066925.1; NP_001159575.1).

Immunogen

Peptide with sequence C-PSAPRRQDSEDH, from the internal region of the protein sequence according to NP_066925.1; NP_001159575.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 19kDa band observed in Human Placenta lysates (calculated MW of 21.8kDa according to NP_001159575.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Testis. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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