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|EB07811||Goat Anti-STRA6 (mouse, rat) Antibody||Pep-ELISA, WB||100µg specific antibody in 200µl||Mouse, Rat||$324.00|
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Peptide with sequence C-QAFRKAALTSAKAN, from the C Terminus of the protein sequence according to NP_033317.2; NP_001025095.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
An approx. 90kDa band was also observed in 3T3-L1 adipocytes reduced by stra6 siRNA. The 90kDa is stained after retrovirally overexpressing Mouse Stra6 and not after overexpressing Human Stra6 in 3T3 preadipocytes (calculated MW of 73.8kDa according to Mouse NP_033317.2). Primary incubation was 3 hours.
This antibody has been successfully used in IF/ ICC on Rat:
Zheng J, He Q, Tang H, Li J, Xu H, Mao X, Liu G
Overexpression of miR-455-5p affects retinol (vitamin A) absorption by downregulating STRA6 in a nitrofen-induced CDH with lung hypoplasia rat model.
Pediatr Pulmonol. 2020 Apr 1.
This antibody has been successfully used in Western blot on Rat:
Zheng J, He Q, Tang H, Xia H
miR‐455‐5p Overexpression Reduces Rat Lung Alveolar Type II Cell Proliferation by Downregulating STRA6
Anat Rec (Hoboken). 2019 May 14.