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Goat Anti-Stromal antigen 2 / STAG2 Antibody (EB09717)

Related Products: EBP07813 - Immunizing Peptide, EBP09717 - Immunizing Peptide, Goat Anti-STAG2 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09717 Goat Anti-Stromal antigen 2 / STAG2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09717 1
EB09717 (0.03µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09717 2
EB09717 (0.3µg/ml) staining of Mouse Spleen (A), Mouse Thymus (B) and Rat Spleen (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: STAG2, stromal antigen 2, DKFZp686P168, DKFZp781H1753, FLJ25871, SA-2, SA2, bA517O1.1, OTTHUMP00000024338, OTTHUMP00000043514, OTTHUMP00000043515, OTTHUMP00000081754, OTTHUMP00000081755, SCC3 homolog 2
Official Symbol: STAG2
Accession Number(s): NP_001036214.1; NP_006594.3
Human GeneID(s): 10735
Non-Human GeneID(s): 20843 (mouse), 313304 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001036214.1; NP_006594.3). Reported variants NP_001036215.1 and NP_001036214.1 represent identical protein: Reported variants NP_001036216.1 and NP_006594.3 represent identical protein.

Immunogen

Peptide with sequence C-SRGSTVRSKKSKPST, from the internal region of the protein sequence according to NP_001036214.1; NP_006594.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 160kDa band observed in lysates of cell line K562 and ~140kDa in rodent Spleen while ~150kDa in Mouse Thymus lysates (calculated MW of 146kDa according to Human NP_001036214.1 and 140kDa according to Mouse NP_001071180.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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