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Goat Anti-STUB1 / CHIP Antibody - Trial (EB05327-T)

Related Products: EBP05327 - Immunizing Peptide, Goat Anti-STUB1 / CHIP Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05327-T Goat Anti-STUB1 / CHIP Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05327-T 1
EB05327 staining (0.3µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB05327-T 2
EB05327 (5µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05327-T 3
EB05327 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: STUB1, CHIP, STIP1 homology and U-Box containing protein 1, HSPABP2, NY-CO-7, SDCCAG7, serologically defined colon cancer antigen 7, carboxy terminus of Hsp70p-interacting protein, heat shock protein A binding protein 2 (c-terminal), LA16c-313D11.6, UBOX1
Official Symbol: STUB1
Accession Number(s): NP_005852.2
Human GeneID(s): 10273

Immunogen

Peptide with sequence C-DAFISENGWVEDY, from the C Terminus of the protein sequence according to NP_005852.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 35kDa band observed in Human Muscle lysates (calculated MW of 34.9kDa according to NP_005852.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was for 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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