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Goat Anti-Synaptotagmin-3 (aa25-38) Antibody (EB11604)

Code Name Applications Availability Size Tested species Price Grade  
EB11604 Goat Anti-Synaptotagmin-3 (aa25-38) Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $255.00 Aspiring

Ordering Information for United States of America (change)

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Target Protein

Principal Names: DKFZp761O132, synaptotagmin III, synaptotagmin-3, SYT3, SytIII
Official Symbol: SYT3
Accession Number(s): NP_115674.1
Human GeneID(s): 84258
Non-Human GeneID(s): 20981 (mouse), 25731 (rat)
Important Comments: Reported variants represent identical protein: NP_001153801.1, NP_001153800.1, NP_115674.1. The immunizing peptide represents part of the vesicular domain.

Immunogen

Peptide with sequence RDADTNDRCQEFN, from the intenal region (near N Terminus) of the protein sequence according to NP_115674.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments in Human, Mouse and Rat Brain lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow