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Goat Anti-Syntenin / SDCBP Antibody (EB07470)

Related Products: EBP07470 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07470 Goat Anti-Syntenin / SDCBP Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07470 1
EB07470 (0.01µg/ml) staining of Human HEK293 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07470 2
EB07470 (10µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SDCBP, Syntenin, syndecan binding protein (syntenin), MDA-9, ST1, SYCL, TACIP18, melanoma differentiation associated protein-9, pro-TGF-alpha cytoplasmic domain-interacting protein 18, scaffold protein Pbp1 syntenin
Official Symbol: SDCBP
Accession Number(s): NP_005616.2; NP_001007068.1; NP_001007069; NP_001007070.1; NP_001007071.1
Human GeneID(s): 6386
Non-Human GeneID(s): 53378 (mouse), 83841 (rat)
Important Comments: This antibody is expected to recognise isoform 1 (NP_005616.2 and NP_001007068.1) and isoform 3 (NP_001007070.1and NP_001007071.1) only.

Immunogen

Peptide with sequence SLEDLKVDKVIQAQ-C, from the N Terminus of the protein sequence according to NP_005616.2; NP_001007068.1; NP_001007069; NP_001007070.1; NP_001007071.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 28-30kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 32.4kDa according to NP_005616.2 and NP_001007068.1 and of 32.3kDa according to NP_001007070.1 and NP_001007071.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Breast. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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