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Goat Anti-TACR1 Antibody (EB07771)

Related Products: EBP07771 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07771 Goat Anti-TACR1 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07771 1
EB07771 (1µg/ml) staining of HeLa (A) and (0.5ug/ml) Neuro2a (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07771 2
EB07771 Immunofluorescence analysis of paraformaldehyde fixed Neuro2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: TACR1, tachykinin receptor 1, NK1R, neurokinin 1 receptor, NKIR, SPR, TAC1R, NK-1 receptor, Tachykinin receptor 1 (substance P receptor,neurokinin-1 receptor), neurokinin 1 receptor, tachykinin 1 receptor (substance P receptor, neurokinin 1 receptor)
Official Symbol: TACR1
Accession Number(s): NP_001049.1*; NP_056542.1*
Human GeneID(s): 6869
Non-Human GeneID(s): 21336 (mouse), 24807 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001049.1 and NP_056542.1).

Immunogen

Peptide with sequence C-EIPGDSSDRYHEQ, from the internal region of the protein sequence according to NP_001049.1*; NP_056542.1*.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 48kDa band observed in lysates of cell lines HeLa and Neuro2a (calculated MW of 46.3kDa according to Human NP_001049.1 and Mouse NP_033339.2 ). Recommended concentration: 0.3-1µg/ml.. Primary incubation 1 hour at room temperature.

Immunofluorescence: Expression of the protein seen in the plasma membrane and nucleus of Neuro2a cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog

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