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Goat Anti-SM22 Alpha / Transgelin Antibody (EB06281)

Code Name Applications Availability Size Tested species Price Grade  
EB06281 Goat Anti-SM22 Alpha / Transgelin Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
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EB06281 1
EB06281 (0.3µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06281 2
EB06281 (5µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: TAGLN, SM22, SMCC, WS3-10, transgelin, SM22-alpha, DKFZp686P11128, TAGLN1, DKFZp686B01212, transgelin variant 2
Official Symbol: TAGLN
Accession Number(s): NP_003177.2
Human GeneID(s): 6876
Non-Human GeneID(s): 21345 (mouse), 25123 (rat)
Important Comments: NP_003177.2 and NP_001001522.1 represent identical protein. The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumour suppressor.

Immunogen

Peptide with sequence C-MTGYGRPRQIIS, from the C Terminus of the protein sequence according to NP_003177.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 23-24kDa band observed in Human Testis lysates (calculated MW of 22.6kDa according to NP_003177.2 and NP_001001522.1). Preliminary testing also showed a band at approx 23kDa in lysates of cell line HeLa and approx 25kDa in Human Breast and Placenta lysates Recommended concentration: 0.1-1 µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific References

This antibody (previous batch) has been successfully used in Western blot on Human:
Caterina Bernacchioni, Tommaso Capezzuoli, Valentina Vannuzzi, Francesca Malentacchi, Francesca Castiglione, Francesca Cencetti, Marcello Ceccaroni, Chiara Donati, Paola Bruni, Felice Petraglia
Sphingosine 1-phosphate receptors are dysregulated in endometriosis: possible implication in transforming growth factor b–induced fibrosis
Fertil Steril. 2020 Sep 6;S0015-0282(20)30766-4.
PMID: 32907751

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Bruno G, Cencetti F, Pertici I, Japtok L, Bernacchioni C, Donati C, Bruni P.
CTGF/CCN2 exerts profibrotic action inmyoblasts via the up-regulation of sphingosine kinase-1/S1P3 signaling axis: Implications in the action mechanism of TGFβ.
Biochim Biophys Acta. 2015 Feb;1851(2):194-202.
PMID: 25457224

This antibody (previous batch) has been successfully used in Western blot on Human:
Donati C, Marseglia G, Magi A, Serratì S, Cencetti F, Bernacchioni C, Nannetti G, Benelli M, Brunelli S, Torricelli F, Cossu G, Bruni P.
Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6.
PLoS One. 2011;6(5):e20389.
PMID: 21629665

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Cencetti F, Bernacchioni C, Nincheri P, Donati C, Bruni P.
Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis.
Mol Biol Cell. 2010 Mar 15;21(6):1111-24.
PMID: 20089836

This antibody (previous batch) has been successfully used in Western blot on Human:
Nincheri P, Luciani P, Squecco R, Donati C, Bernacchioni C, Borgognoni L, Luciani G, Benvenuti S, Francini F, Bruni P.
Sphingosine 1-phosphate induces differentiation of adipose tissue-derived mesenchymal stem cells towards smooth muscle cells.
Cell Mol Life Sci. 2009 May;66(10):1741-54.
PMID: 19337690

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