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Code Name Applications Availability Size Tested species Price Grade  
EB08220 Goat Anti-Tankyrase 2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08220 1
EB08220 (0.5µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TNKS2, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2, PARP-5b, PARP-5c, PARP5B, PARP5C, TANK2, TNKL, tankyrase 2
Official Symbol: TNKS2
Accession Number(s): NP_079511.1
Human GeneID(s): 80351
Non-Human GeneID(s): 74493 (mouse)

Immunogen

Peptide with sequence C-HRRKEVSEENHNH, from the internal region of the protein sequence according to NP_079511.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 125 kDa band observed in lysates of cell line Jurkat (calculated MW of 127 kDa according to NP_079511.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 45 kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

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