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Goat Anti-TDP-43 Antibody (EB09108)

Code Name Applications Availability Size Tested species Price Grade  
EB09108 Goat Anti-TDP-43 Antibody Pep-ELISA, WB Allow up to 10 days for delivery 100µg specific antibody in 200µl Human $423.00 Elite

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EB09108 1
EB09108 (0.3ug/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09108 2
EB09108 (1µg/ml) staining of Human Cerebellum (A), and Hippocampus (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Target Protein

Principal Names: TARDBP, TAR DNA binding protein, ALS10, TDP-43, OTTHUMP00000002171, TAR DNA-binding protein-43
Official Symbol: TARDBP
Accession Number(s): NP_031401.1
Human GeneID(s): 23435
Non-Human GeneID(s): 230908 (mouse)

Immunogen

Peptide with sequence C-HISNAEPKHNSNRQ, from the internal region of the protein sequence according to NP_031401.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 45kDa band observed in lysates of cell line Jurkat and in Human Cerebellum lysates and approx. 48kDa in Hippocampus lysates (calculated MW of 44.7kDa according to NP_031401.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow