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Goat Anti-TFF3 Antibody (EB12315)

Code Name Applications Availability Size Tested species Price Grade  
EB12315 Goat Anti-TFF3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

Ordering Information for United States of America (change)

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EB12315 1
EB12315 (1µg/ml) staining of Colon cancer lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TFF3, trefoil factor 3 (intestinal), ITF, P1B, TFI, polypeptide P1.B, trefoil factor 3
Official Symbol: TFF3
Accession Number(s): NP_003217.3
Human GeneID(s): 7033

Immunogen

Peptide with sequence SANQCAVPAKDRVD, from the internal region of the protein sequence according to NP_003217.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 13kDa band observed in Human Colon cancer, and in normal Duodenum and Ileum lysates (calculated MW of 10.2kDa according to NP_003217.3). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human