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Goat Anti-TGFBR1 (aa50-63) Antibody (EB11053)

Related Products: EBP11053 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11053 Goat Anti-TGFBR1 (aa50-63) Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human, Rat $464.00 Elite_Plus

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EB11053 1
EB11053 (0.1µg/ml) staining of Rat Liver (A) and (0.03ug/ml) Lung (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11053 2
EB11053 (5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB11053 3
EB11053 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB11053 4
EB11053 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TGFBR1, transforming growth factor, beta receptor 1, AAT5, ACVRLK4, ALK-5, ALK5, LDS1A, LDS2A, SKR4, TGFR-1, TGF-beta receptor type I, TGF-beta receptor type-1, TGF-beta type I receptor, activin A receptor type II-like kinase, 53kD, activin A receptor type II-like kinase, 53kDa, activin receptor-like kinase 5, serine/threonine-protein kinase receptor R4, tbetaR-I, transforming growth factor beta receptor I, transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kD), transforming growth factor-beta receptor type I, AAT5, activin A receptor type II-like kinase, 53kD, activin A receptor type II-like kinase, 53kDa, activin receptor-like kinase 5, ACVRLK4, ALK5, ALK-5, LDS1A, LDS2A, serine/threonine-protein kinase receptor R4, SKR4, tbetaR-I, TGF-beta receptor type I, TGF-beta receptor type-1, TGF-beta type I receptor, TGFR-1, transforming growth factor beta receptor I, transforming growth factor, beta receptor 1, transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kD), transforming growth factor-beta receptor type I, TGFBR1
Official Symbol: TGFBR1
Accession Number(s): NP_004603.1; NP_001124388.1
Human GeneID(s): 7046
Non-Human GeneID(s): 21812 (mouse), 29591 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004603.1; NP_001124388.1). The immunizing peptide represents part of a potential extracellular domain.

Immunogen

Peptide with sequence TDGLCFVSVTETTD, from the internal region of the protein sequence according to NP_004603.1; NP_001124388.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55kDa band observed in Rat Liver and Rat Lung lysates (calculated MW of 55.6kDa according to Rat NP_036907.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Adrenal Gland and Small Intestine. Recommended concentration: 5µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Immunofluorescence: This antibody is not recommended for IF testing.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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