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Goat Anti-TIAL1 & TIA Antibody (EB09793)

Code Name Applications Availability Size Tested species Price Grade  
EB09793 Goat Anti-TIAL1 & TIA Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
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EB09793 1
EB09793 (2µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09793 2
EB09793 (3.8µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MGC33401, OTTHUMP00000020602, OTTHUMP00000020605, TCBP, T-cluster binding protein, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TIA1 cytotoxic granule-associated RNA-binding p, TIA-1 related protein, TIA-1-related nucleolysin, TIAR, TIAL1
Official Symbol: TIAL1
Accession Number(s): NP_003243.1; NP_001029097.1
Human GeneID(s): 7073 , 7072
Non-Human GeneID(s): 21843 (mouse)
Important Comments: This antibody is expected to recognize both isoforms (NP_003243.1; NP_001029097.1).


Peptide with sequence C-SPEITTEDIKSAFAP, from the internal region of the protein sequence according to NP_003243.1; NP_001029097.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 45kDa band observed in lysates of cell line K562 (calculated MW of 43.4kDa according to NP_001029097.1). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Skin shows staining of select keratinocytes. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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