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Goat Anti-TIPRL Antibody (EB09794)

Related Products: EBP09794 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09794 Goat Anti-TIPRL Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09794 1
EB09794 (1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09794 2
EB09794 (3.75µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: CG9578-like, dJ69E11.3, MGC3794, OTTHUMP00000032581, OTTHUMP00000032582, putative MAPK activating protein, TIP, TIP41, TIP41, TOR signaling pathway regulator-like (S. cerevisiae), TIP41, TOR signalling pathway regulator-like, yeast YPR037W and worm, TIPRL
Official Symbol: TIPRL
Accession Number(s): NP_690866.1
Human GeneID(s): 261726
Non-Human GeneID(s): 226591 (mouse), 360869 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_690866.1).

Immunogen

Peptide with sequence C-RIDPNPADSQKSTQ, from the C Terminus of the protein sequence according to NP_690866.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 30kDa band observed in lysates of cell line K562 (calculated MW of 31.4kDa according to NP_690866.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Breast. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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