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Goat Anti-TLR6 Antibody (EB10208)

Related Products: EBP10208 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10208 Goat Anti-TLR6 Antibody Pep-ELISA, FC, IF Yes 100µg specific antibody in 200µl Human $423.00 Elite

Ordering Information for United States of America (change)

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EB10208 1
EB10208 Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB10208 2
EB10208 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic (endoplasmic reticulum) staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB10208 3
EB10208 Flow cytometric analysis of paraformaldehyde fixed THP-1 cells (blue line) permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488® conjugated secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CD286, OTTHUMP00000158730, toll-like receptor 6, TLR6
Official Symbol: TLR6
Accession Number(s): NP_006059.2
Human GeneID(s): 10333

Immunogen

Peptide with sequence C-RGSTLLNFTLNH, from the internal region of the protein sequence according to NP_006059.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.

Immunofluorescence: Strong expression of the protein seen in THP-1 and A549 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow, Pig, Sheep