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Code Name Applications Availability Size Tested species Price Grade  
EB08297 Goat Anti-TMPRSS4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08297 1
EB08297 (0.5µg/ml) staining lysates of cell line HEK293 (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: transmembrane protease, serine 4, MT-SP2, TMPRSS3, membrane-type serine protease 2, transmembrane serine protease 3, type II membrane serine protease
Official Symbol: TMPRSS4
Accession Number(s): NP_063947.1; NP_001077416.1
Human GeneID(s): 56649
Non-Human GeneID(s): 214523 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_063947.1 and NP_001077416.1).

Immunogen

Peptide with sequence DSTRCNADDAYQGE, from the internal region of the protein sequence according to NP_063947.1; NP_001077416.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48kDa band observed in lysates of cell line HEK293 (calculated MW of 48.2kDa according to NP_063947.1 and of 47.6kDa according to NP_001077416.1). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

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