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Goat Anti-TNNT3, Biotinylated Antibody (EB12033-B)

Related Products: EBP12033-B - Immunizing Peptide, EBP12033 - Immunizing Peptide, Goat Anti-TNNT3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB12033-B Goat Anti-TNNT3, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12033-B 1
Biotinylated EB12033 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: TNNT3, troponin T3, fast skeletal type, TNTF, beta TnTF, beta-TnTF, fTnT, fast skeletal muscle troponin T, troponin T type 3 (skeletal, fast), troponin-T3, skeletal, fast
Official Symbol: TNNT3
Accession Number(s): NP_006748.1; NP_001036246.1; NP_001036247.1
Human GeneID(s): 7140
Important Comments: This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1). Reported variants represent identical protein: NP_001284575.1, NP_001036247.1

Immunogen

Peptide with sequence CTTLRSRIDQAQKHSK., from the C Terminus of the protein sequence according to NP_006748.1; NP_001036246.1; NP_001036247.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 35kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6kDa according to NP_006748.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et al, Circ Res. 1997 Jul;81(1):76-85. PMID: 9201030.).Recommended concentration: 0.01-0.03µg/ml. See non-biotinylated parental product's datasheet for further QC data.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

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