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Goat Anti-Transcortin / CBG (rat) Antibody (EB10756)

Related Products: EBP10756 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10756 Goat Anti-Transcortin / CBG (rat) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10756 1
EB10756 (0.03µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Serpina6, serine (or cysteine) peptidase inhibitor, clade A, member 6, MGC112780, Cbg, corticosteroid binding globulin, corticosteroid-binding globulin, serine (or cysteine) proteinase inhibitor, clade A, (alpha-1 antiproteinase, antitrypsin) member 6, serpin A6, transcortin
Official Symbol: Serpina6
Accession Number(s): NP_001009663.1
Non-Human GeneID(s): 299270 (rat)

Immunogen

Peptide with sequence C-KASQQINQHVKDKT, from the internal region of the protein sequence according to NP_001009663.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 55kDa band observed in Rat Liver lysates (calculated MW of 44.7kDa according to NP_001009663.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Richard et al, Endocrinology. 2010 Feb;151(2):649-59; PMID: 20022933). Recommended concentration: 0.03-0.1µg/ml.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Rat

Specific Reference

Romero Mdel M, Holmgren-Holm F, Grasa Mdel M, Esteve M, Remesar X, Fernández-López JA, Alemany M.
Modulation in Wistar rats of blood corticosterone compartmentation by sex and a cafeteria diet.
PLoS One. 2013;8(2):e57342.
PMID: 23451210

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