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Goat Anti-Transcription factor E2F4 Antibody (EB12262)

Related Products: EBP09937 - Immunizing Peptide, EBP12262 - Immunizing Peptide, Goat Anti-E2F4 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB12262 Goat Anti-Transcription factor E2F4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Pig $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12262 1
EB12262 (0.3µg/ml) staining of A43 (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12262 2
EB12262 (0.003µg/ml) staining of Pig Brain (A) and Heart (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Click here to download PDF datasheet.

Target Protein

Principal Names: E2F4, E2F transcription factor 4, p107/p130-binding, E2F-4, p107/p130-binding protein, transcription factor E2F4
Official Symbol: E2F4
Accession Number(s): NP_001941.2
Human GeneID(s): 1874
Non-Human GeneID(s): 104394 (mouse), 100360427 (rat)

Immunogen

Peptide with sequence C-PKELSEIFDPTR, from the internal region of the protein sequence according to NP_001941.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 50kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 44.0kDa according to NP_001941.2). Recommended concentration: 0.1-0.3µg/ml. Approx 40+50kDa bands observed in Pig Brain and Heart lysates (calculated MW of 43.2kDa according to Pig XP_003126981.3). Recommended concentration: 0.01-0.003µg/ml.

Species Reactivity

Tested: Human, Pig
Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow

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