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Goat Anti-TRIM29 / ATDC Antibody (EB09814)

Related Products: EBP09814 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09814 Goat Anti-TRIM29 / ATDC Antibody Pep-ELISA, WB, IF, FC, IHC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09814 1
EB09814 (1µg/ml) staining of Peripheral Blood Mononucleocyte (A) and (0.1 ug/ml) HeLa (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09814 2
EB09814 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and Intermediate filament staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB09814 3
EB09814 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB09814 4
EB09814 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB09814 5
EB09814 (3.75µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: ataxia-telangiectasia group D-associated protein, ATDC, FLJ36085, tripartite motif protein TRIM29, tripartite motif-containing 29, TRIM29
Official Symbol: TRIM29
Accession Number(s): NP_036233.2; NP_001317311.1
Human GeneID(s): 23650
Non-Human GeneID(s): 72169 (mouse), 300656 (rat)

Immunogen

Peptide with sequence C-EKDRIKSFTTNE, from the internal region of the protein sequence according to NP_036233.2; NP_001317311.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70kDa band was seen in some PBM lysates (calculated MW of 37.3kDa according to NP_001317311.1 (isoform 2) and 65.8kDa according to NP_036233.2 (isoform 1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room tempetature.
IHC: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 3.75µg/ml.

Immunofluorescence: Strong expression of the protein seen in the Cytoplasm and Intermediate filaments of A431 cells. Strong expression of the protein seen in the Nucleus of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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