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Goat Anti-TRIM71 (aa409-419) Antibody (EB09902)

Related Products: EBP09901 - Immunizing Peptide, EBP09902 - Immunizing Peptide, Goat Anti-TRIM71 (aa390-402) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09902 Goat Anti-TRIM71 (aa409-419) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09902 1
EB09902 (0.5µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: abnormal cell LINeage LIN-41, homolog of C. elegans Lin-41, LIN41, LIN-41, tripartite motif-containing 71, TRIM71
Official Symbol: TRIM71
Accession Number(s): NP_001034200.1
Human GeneID(s): 131405
Non-Human GeneID(s): 636331 (mouse), 301042 (rat)

Immunogen

Peptide with sequence C-NKLESTISAVQ , from the internal region of the protein sequence according to NP_001034200.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 93.4kDa according to Human NP_001034200.1 and of 92.1kDa according to Mouse NP_001035968.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 14kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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