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Code Name Applications Availability Size Tested species Price Grade  
EB10443 Goat Anti-TRIM72 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB10443 1
EB10443 (0.01µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10443 2
EB10443 (3.8µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: tripartite motif-containing 72, TRIM72
Official Symbol: TRIM72
Accession Number(s): NP_001008275.1
Human GeneID(s): 493829
Non-Human GeneID(s): 434246 (mouse), 365377 (rat)

Immunogen

Peptide with sequence C-RPQALSTNLQ, from the internal region of the protein sequence according to NP_001008275.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 52kDa band observed in Mouse Heart lysates (calculated MW of 52.8kDa according to Mouse NP_001073401.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Skeletal Muscle shows staining of the fibre membranes, detached artificially by the sectioning. Recommended concentration: 3-5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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