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Goat Anti-TRPM7 / LTRPC7 Antibody - Trial (EB05129-T)

Related Products: EBP05129 - Immunizing Peptide, Goat Anti-TRPM7 / LTRPC7 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05129-T Goat Anti-TRPM7 / LTRPC7 Antibody - Trial Pep-ELISA, IHC, IF Yes 20µg specific antibody in 40µl Human, Mouse $105.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
TestShot
EB05129-T 1
EB05129 (4µg/ml) staining of paraffin embedded Mouse Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.
EB05129-T 2
EB05129 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: TRPM7, transient receptor potential cation channel, subfamily M, member 7, CHAK, CHAK1, LTRPC7, FLJ20117, TRP-PLIK, LTRPC ion channel family member 7, homolog of mouse transient receptor potential-phospholipase C-interacting kinase CHaK hypothetical protein FLJ20117, FLJ25718
Official Symbol: TRPM7
Accession Number(s): NP_060142.3; NP_001288141.1
Human GeneID(s): 54822

Immunogen

Peptide with sequence C-TKESESTNSVRLML, from the C Terminus of the protein sequence according to NP_060142.3; NP_001288141.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
IHC: In paraffin embedded Mouse Brain shows neuronal staining in the Hippocampus. Recommended concentration: 4-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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