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Goat Anti-TRPM7 / LTRPC7 Antibody - Trial (EB05129-T)

Code Name Applications Availability Size Tested species Price Grade  
EB05129-T Goat Anti-TRPM7 / LTRPC7 Antibody - Trial Pep-ELISA, IHC, IF Yes 20µg specific antibody in 40µl Human $105.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

TestShot
EB05129-T 1
EB05129 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05129-T 2
EB05129 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05129-T 3
EB05129 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05129-T 4
EB05129 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRPM7, transient receptor potential cation channel, subfamily M, member 7, CHAK, CHAK1, LTRPC7, FLJ20117, TRP-PLIK, LTRPC ion channel family member 7, homolog of mouse transient receptor potential-phospholipase C-interacting kinase CHaK hypothetical protein FLJ20117, FLJ25718
Official Symbol: TRPM7
Accession Number(s): NP_060142.3; NP_001288141.1
Human GeneID(s): 54822

Immunogen

Peptide with sequence C-TKESESTNSVRLML, from the C Terminus of the protein sequence according to NP_060142.3; NP_001288141.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in A431 and MCF7 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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