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Goat Anti-Tryptophan hydroxylase 2 / TPH2 Antibody (EB07050)

Code Name Applications Availability Size Tested species Price Grade  
EB07050 Goat Anti-Tryptophan hydroxylase 2 / TPH2 Antibody Pep-ELISA, IHC, IF Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB07050 1
EB07050 (4µg/ml) staining of paraffin embedded Human Brain Stem. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TPH2, tryptophan hydroxylase 2, HGNC:20692, FLJ37295, NTPH, neuronal tryptophan hydroxylase, ADHD7, MGC138871, MGC138872, tryptophan 5-monooxygenase 2
Official Symbol: TPH2
Accession Number(s): NP_775489.2
Human GeneID(s): 121278

Immunogen

Peptide with sequence C-NKPNSGKNDDKGNK, from the internal region of the protein sequence according to NP_775489.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments in human brain lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC: In paraffin embedded Human Brain Stem shows layered neuronal staining. Recommended concentration: 4-6µg/ml.

Immunofluorescence:This product has been successfully used in IF on Mouse: Rahim RS et al, Mol Cell Neurosci. 2018 Apr;88:16-32. PMID: 29187321, and Rahim RS et al, Neuroscience 334 (2016) 201-213. PMID: 27514574.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Dog, Pig, Cow

Specific References

This antibody has been successfully used in IF on Mouse:
Rahim RS, St John JA, Crane DI, Meedeniya ACB
Impaired neurogenesis and associated gliosis in mouse brain with PEX13 deficiency.
Mol Cell Neurosci. 2018 Apr;88:16-32
PMID: 29187321

This antibody has been successfully used in IF in Mouse:
Rani Sadia Rahim, Mo Chen, C. Cathrin Nourse, Adrian C. B. Meedeniya and Denis I. Crane.
Mitochondrial Changes and Oxidative Stress in a Mouse Model of Zellweger Syndrome Neuropathogenesis.
Neuroscience 334 (2016) 201–213.
PMID: 27514574

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