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Goat Anti-TSPAN5 / NET-4 Antibody (EB11778)

Related Products: EBP11778 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11778 Goat Anti-TSPAN5 / NET-4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $423.00 Elite

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EB11778 1
EB11778 (0.3µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NET4, NET-4, tetraspan 5, tetraspan NET-4, tetraspan TM4SF, tetraspanin 5, tetraspanin-5, TM4SF9, transmembrane 4 superfamily member 9, transmembrane 4 superfamily, member 8, TSPAN5, TSPAN-5
Official Symbol: TSPAN5
Accession Number(s): NP_005714.2
Human GeneID(s): 10098
Non-Human GeneID(s): 56224 (mouse), 362048 (rat)

Immunogen

Peptide with sequence SGKHYKGPEVSC, from the N Terminus of the protein sequence according to NP_005714.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 35kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 30.3kDa according to NP_005714.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat Dog, Pig, Cow

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