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Goat Anti-TUSC3 / N33 Antibody (EB08380)

Code Name Applications Availability Size Tested species Price Grade  
EB08380 Goat Anti-TUSC3 / N33 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB08380 1
EB08380 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08380 2
EB08380 (8µg/ml) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB08380 3
EB08380 Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TUSC3, tumor suppressor candidate 3, D8S1992, MGC13453, N33, OST3A, Putative prostate cancer tumor suppressor
Official Symbol: TUSC3
Accession Number(s): NP_006756.2; NP_839952.1
Human GeneID(s): 7991
Non-Human GeneID(s): 80286 (mouse), 290783 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_006756.2; NP_839952.1).

Immunogen

Peptide with sequence PQRQCSVCRQAN, from the internal region of the protein sequence according to NP_006756.2; NP_839952.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 37kDa band observed in Human Liver and Lung lysates (calculated MW of 39.7kDa according to NP_006756.2 and of 39.6 according to NP_839952.1). An additional band of unknown identity was also consistently observed at 22kDa, which was successfully blocked by incubation with the immunising peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Prostate. Recommended concentration: 4-8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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