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Goat Anti-UBC3B Antibody (EB05752)

Related Products: EBP05752 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05752 Goat Anti-UBC3B Antibody Pep-ELISA, IEM, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05752 1
EB05752 (2µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBE2R2, UBC3B, CDC34B, FLJ20419, MGC10481, ubiquitin-conjugating enzyme E2R 2, ubiquitin-conjugating enzyme UBC3B
Official Symbol: UBE2R2
Accession Number(s): NP_060281
Human GeneID(s): 54926
Non-Human GeneID(s): 67615 (mouse)

Immunogen

Peptide with sequence AQQQMTSSQKALM-C, from the N Terminus of the protein sequence according to NP_060281.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Testis shows textured nuclear staining of the primary spermatocytes . Recommended concentration, 2-4µg/ml.

Immunoelectron microscopy: This product stained chromatoid bodies in Rat spermatids.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat

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