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Goat Anti-UBE2L3 Antibody - Trial (EB05770-T)

Related Products: EBP05770 - Immunizing Peptide, Goat Anti-UBE2L3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05770-T Goat Anti-UBE2L3 Antibody - Trial Pep-ELISA, WB, IF Yes 20µg specific antibody in 40µl Human, Mouse, Rat, Pig $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05770-T 1
EB05770 staining (0.5µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05770-T 2
EB05770 (0.01µg/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05770-T 3
EB05770 (0.03µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05770-T 4
EB05770 (0.01µg/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05770-T 5
Immunofluorescence staining of MCF7 (A) and HeLa (B) cells with 5ug/ml EB05770 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.
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Target Protein

Principal Names: UBE2L3, ubiquitin-conjugating enzyme E2L 3, UBCH7, E2-F1, L-UBC, UbcM4, ubiquitin carrier protein, ubiquitin-conjugating enzyme UBCH7, ubiquitin-protein ligase
Official Symbol: UBE2L3
Accession Number(s): NP_003338.1
Human GeneID(s): 7332
Non-Human GeneID(s): 22195 (mouse)

Immunogen

Peptide with sequence C-EFTKKYGEKRPVD, from the C Terminus of the protein sequence according to NP_003338.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 18kDa band observed in lysates of cell lines HeLa HEK293, HepG2 and Jurkat and also in lysates of Human, Mouse and Rat Heart, Skeletal Muscle and Testis and of Pig Heart (calculated MW of 17.9kDa according to Human NP_003338.1 and Mouse NP_033482.1). Recommended concentration: 1-3µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm. Dividing cells appear to show enhanced expression. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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