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Goat Anti-ULK3 (aa445-458) Antibody (EB11184)

Related Products: EBP11184 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11184 Goat Anti-ULK3 (aa445-458) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB11184 1
EB11184 (1µg/ml) staining of Human Hippocampus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DKFZp434C131, FLJ90566, serine/threonine-protein kinase ULK3, unc-51-like kinase 3 (C. elegans), ULK3
Official Symbol: ULK3
Accession Number(s): NP_001092906.1
Human GeneID(s): 25989

Immunogen

Peptide with sequence C-KMRESRWEADTLDK, from the C Terminus of the protein sequence according to NP_001092906.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50kDa band observed in Human Brain (Cerebellum, Cerebral Cortex, Hippocampus) lysates (calculated MW of 53.4kDa according to NP_001092906.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig, Dog

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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