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Goat Anti-Ulk3 (mouse) Antibody (EB11185)

Related Products: EBP11185 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11185 Goat Anti-Ulk3 (mouse) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB11185 1
EB11185 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: 1200015E14Rik, serine/threonine-protein kinase ULK3, unc-51-like kinase 3 (C. elegans), ULK3
Official Symbol: Ulk3
Accession Number(s): NP_082171.1
Non-Human GeneID(s): 71742 (mouse), 691171 (rat)

Immunogen

Peptide with sequence C-KIRESHWEAESLDK, from the C Terminus of the protein sequence according to NP_082171.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 47kDa band in Rat Brain and Eye lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.6kDa according to Mouse NP_001092906.1 and Rat XP_001077085.2. The 47kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Mouse, Rat

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