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Goat Anti-UNC5C Antibody (EB09218)

Related Products: EBP09218 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09218 Goat Anti-UNC5C Antibody Pep-ELISA, IF, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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www.arp1.com
EB09218 1
EB09218 (50ug/ml) staining of islets in Mouse Pancreas. Citrate antigen retrieval. Detected by immunofluorescence.
EB09218 2
EB09218 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: UNC5C, unc-5 homolog C (C. elegans), UNC5H3, homolog of C. elegans transmembrane receptor Unc5, unc5 (C.elegans homolog) c, unc5C
Official Symbol: UNC5C
Accession Number(s): NP_003719.2
Human GeneID(s): 8633
Non-Human GeneID(s): 22253 (mouse)

Immunogen

Peptide with sequence NCTVSEEPTGID, from the internal region of the protein sequence according to NP_003719.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in Human and Mouse Brain lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml).
IHC: In paraffin embedded Human Cerebral Cortex shows staining of some neuronal bodies plus intense axonal staining. Recommended concentration: 3-5µg/ml.

Immunofluorescence: Staining of paraffin embedded endocrine islets (red) in adult Mouse Pancreas. Nuclear staining in blue and ductal cell staining in green. Data obtained from a customer.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog

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