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Goat Anti-Uromodulin (aa525-536) Antibody (EB11647)

Related Products: EBP11647 - Immunizing Peptide, EBP11648 - Immunizing Peptide, Goat Anti-Uromodulin Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11647 Goat Anti-Uromodulin (aa525-536) Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB11647 1
EB11647 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: ADMCKD2, FJHN, HNFJ, HNFJ1, MCKD2, OTTHUMP00000162212, OTTHUMP00000162213, Tamm-Horsfall glycoprotein, tamm-Horsfall urinary glycoprotein, THGP, THP, UMOD, uromodulin, uromodulin (uromucoid, Tamm-Horsfall glycoprotein), uromucoid
Official Symbol: UMOD
Accession Number(s): NP_003352.2
Human GeneID(s): 7369
Important Comments: Reported variants represent identical protein: NP_001008390.1, NP_003352.2

Immunogen

Peptide with sequence DRCPHTRDSTIQ, from the internal region of the protein sequence according to NP_003352.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.

Immunofluorescence: Strong expression of the protein seen in the Plasma membrane and Golgi of HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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