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Goat Anti-USH1C / Harmonin Antibody (EB06287)

Related Products: EBP06287 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06287 Goat Anti-USH1C / Harmonin Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06287 1
EB06287 (0.1µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: USH1C, harmonin, AIE-75, DFNB18, PDZ-45, PDZ-73, HARMONIN, NY-CO-37, NY-CO-38, PDZ-73/NY-CO-38, Usher syndrome 1C (autosomal recessive, severe), PDZ-73 protein, ush1cpst, deafness, autosomal recessive 18
Official Symbol: USH1C
Accession Number(s): NP_005700.2; NP_710142.1
Human GeneID(s): 10083
Non-Human GeneID(s): 72088 (mouse), 308596 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005700.2 and NP_710142.1).

Immunogen

Peptide with sequence DRKVAREFRHKVD-C, from the N Terminus of the protein sequence according to NP_005700.2; NP_710142.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 75kDa band observed in lysates of cell line HEK293 (calculated MW of 62.2kDa according to NP_005700.2). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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