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Goat Anti-VDR Antibody - Trial (EB06803-T)

Related Products: EBP06803 - Immunizing Peptide, Goat Anti-VDR Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06803-T Goat Anti-VDR Antibody - Trial Pep-ELISA, WB, FC Yes 20µg specific antibody in 40µl Human $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB06803-T 1
EB06803 (3µg/ml) staining of U937 cell lysate (35µg protein in RIPA buffer. Detected by chemiluminescence.
EB06803-T 2
EB06803 (3µg/ml) staining of Human Placenta (A) and (2µg/ml) Duodenum (B) lysate (35µg protein in RIPA buffer. Detected by chemiluminescence.
EB06803-T 3
EB06803 Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: VDR, vitamin D (1,25- dihydroxyvitamin D3) receptor, HGNC:12679, NR1I1, vitamin D (1,25-dihydroxyvitamin D3) receptor
Official Symbol: VDR
Accession Number(s): NP_000367.1; NP_001017535.1; NP_001351014.1;NP_001017536.1
Human GeneID(s): 7421
Non-Human GeneID(s): 22337 (mouse), 24873 (rat)

Immunogen

Peptide with sequence CGNQDYKYRVSD, from the internal region of the protein sequence according to NP_000367.1; NP_001017535.1; NP_001351014.1;NP_001017536.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 55kDa band observed in Human Placenta and Duodenum lysates and approx. 55-60kDa in lysates of cell line U937 (calculated MW of 55.2kDa according to NP_001351014.1). Recommended concentration: 2-3µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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