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Goat Anti-VGLUT2 (aa521-534) Antibody (EB12130)

Code Name Applications Availability Size Tested species Price Grade  
EB12130 Goat Anti-VGLUT2 (aa521-534) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB12130 1
EB12130 (2µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SLC17A6, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, DNPI, VGLUT2, differentiation-associated BNPI, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, differentiation-associated Na-dependent inorganic phosphate cotransporter, solute carrier family 17 member 6, vesicular glutamate transporter 2
Official Symbol: SLC17A6
Accession Number(s): NP_065079.1
Human GeneID(s): 57084
Non-Human GeneID(s): 140919 (mouse), 84487 (rat)

Immunogen

Peptide with sequence C-EDELDEETGDITQN, from the internal region of the protein sequence according to NP_065079.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 60kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 64.4kDa according to NP_065079.1. Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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