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Goat Anti-VHL, Biotinylated Antibody (EB07333-B)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07333-B | Goat Anti-VHL, Biotinylated Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human, Mouse, Rat | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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Biotinylated EB07333 (0.3µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Target Protein
Principal Names: VHL, von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase, HRCA1, RCA1, VHL1, pVHL, elongin binding protein, protein G7Official Symbol: VHL
Accession Number(s): NP_000542.1; NP_937799.1; XP_011532380.1
Human GeneID(s): 7428
Non-Human GeneID(s): 22346 (mouse), 24874 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).
Immunogen
Peptide with sequence C-RSLVKPENYRRLD., from the internal region of the protein sequence according to NP_000542.1; NP_937799.1; XP_011532380.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 17kDa band observed in Human Ovary lysates (calculated MW of 19.7kDa according to NP_937799.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.
Species Reactivity
Tested: Human, Mouse, RatExpected from sequence similarity: Human