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Goat Anti-VHL, Biotinylated Antibody (EB07333-B)

Related Products: EBP07333-B - Immunizing Peptide, EBP07333 - Immunizing Peptide, Goat Anti-VHL Antibody, Goat Anti-VHL Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB07333-B Goat Anti-VHL, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07333-B 1
Biotinylated EB07333 (0.3µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: VHL, von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase, HRCA1, RCA1, VHL1, pVHL, elongin binding protein, protein G7
Official Symbol: VHL
Accession Number(s): NP_000542.1; NP_937799.1; XP_011532380.1
Human GeneID(s): 7428
Non-Human GeneID(s): 22346 (mouse), 24874 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).

Immunogen

Peptide with sequence C-RSLVKPENYRRLD., from the internal region of the protein sequence according to NP_000542.1; NP_937799.1; XP_011532380.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 17kDa band observed in Human Ovary lysates (calculated MW of 19.7kDa according to NP_937799.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human

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