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Goat Anti-VMAT2 / SLC18A2 Antibody (EB06558)

Code Name Applications Availability Size Tested species Price Grade  
EB06558 Goat Anti-VMAT2 / SLC18A2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $324.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
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Ph. (800) 832-2611
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EB06558 1
EB06558 (2µg/ml) staining of Human Adrenal Gland (A) and Mouse Brain (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06558 2
EB06558 (5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB06558 3
EB06558 (2.5µg/ml) staining of paraffin embedded Human Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.This data is from a previous batch, not on sale.
EB06558 4
EB06558 Negative Control showing staining of paraffin embedded Human Adrenal Gland, with no primary antibody. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: VMAT2, SLC18A2, SVAT, SVMT, VAT2, solute carrier family 18 (vesicular monoamine), member 2
Official Symbol: SLC18A2
Accession Number(s): NP_003045.2
Human GeneID(s): 6571
Non-Human GeneID(s): 214084 (mouse), 25549 (rat)

Immunogen

Peptide with sequence C-SYPIGEDEESESD, from the C Terminus of the protein sequence according to NP_003045.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55-58kDa band observed in Human Adrenal Gland and approx. 50kDa in Mouse Brain lysates (calculated MW of 55.7kDa according to Human NP_003045.2 and Mouse NP_766111.1). Recommended concentration 1-3ug/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific References

This antibody (previous batch) has been successfully used in IF on Rat:
Kato K, Yokoyama T, Kusakabe T, Hata K, Fushuku S, Nakamuta N, Yamamoto Y
Differences in the expression of catecholamine-synthesizing enzymes between vesicular monoamine transporter 1- and 2-immunoreactive glomus cells in the rat carotid body
Acta Histochem. 2020 Jan 16:151507.
PMID: 31955909

This antibody (previous batch) has been successfully used in Western blot on Rat:
Shiliang Zhang, Jia Qi, Xueping Li, Hui-Ling Wang, Jonathan P. Britt, Alexander F. Hoffman, Antonello Bonci, Carl R. Lupica, and Marisela Morales.
Dopaminergic and glutamatergic microdomains within a subset of rodent mesoaccumbens axons.
Nat Neurosci. 2015 March;18(3):386–392.
PMID: 25664911

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