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Code Name Applications Availability Size Tested species Price Grade  
EB12493 Goat Anti-VPS16 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12493 1
EB12493 (1µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB12493 2
EB12493 (0.5µg/ml) staining of Mouse Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: VPS16, vacuolar protein sorting 16 homolog (S. cerevisiae), hVPS16, OTTHUMP00000030047, OTTHUMP00000030048, vacuolar protein sorting protein 16, vacuolar protein sorting-associated protein 16 homolog
Official Symbol: VPS16
Accession Number(s): NP_072097.2; NP_536338.1
Human GeneID(s): 64601
Non-Human GeneID(s): 80743 (mouse), 296159 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_072097.2; NP_536338.1).

Immunogen

Peptide with sequence CYKVQQKDVSDED, from the internal region of the protein sequence according to NP_072097.2; NP_536338.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 95+80kDa band observed in lysates of cell line HepG2 and 95kDa in Mouse Spleen (calculated MW of 94.7kDa according to NP_072097.2 and 78.3kDa according to NP_536338.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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